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KMID : 0545120010110020259
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 2 p.259 ~ p.265
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Antibacterial Effect of the Surface-Modified Biomedical Polyurethane against Staphylococcus aureus and Staphylococcus epidermidis
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Jeon, Sung Min
Kim, Hyun Jung/Lee, Kyu Back/Kim, Jong Won/Kim, Mal Nam
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Abstract
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Staphylococcal infection still remains to be one of the most serious infections, having various complications in the clinical use of indwelling polymeric medical devices. However, there are a few promising systems showing a high antibacterial effect without causing any damage of polymer backbone under biological environments such as blood or body fluid. In order to resolve this problem, we have designed a new antibiotic releasing system via a hydrolysis mechanism. The surface of biomedical polyurethane (PU) was modified by using 1,6-diisocyanatohexane (HMDI) to immobilize the rifampicin. Also, the immobilized rifampicin was designed to be released by a selective cleavage of the unstable carbamate linkage that exists on the rifampicin-immobilized polyurethane (PHR). The immobilization of rifampicin on the surface of polyurethane was confirmed by the disappearance of the characteristic IR absorbance peak of the isocyanate (-NCO) group at 2,267㎝^-1. The PHR showed a continuous rifampicin release profile under an aqueous environment of 10mM of PBS (phosphate-buffered saline) for over 6 days. The rifampicin molecules, which are released from PHR under an optimal bacterial infection environment, had a higher antibacterial activity against both S. aureus and S. epidermidis than rifampicin-incorporated polyurethane (RIP). In addition, the PHR maintained a stable antibacterial effect under a bloodmimic aqueous environment such as bovine calf serum.
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